Abrogation of resistance to and enhancement of DLA-nonidentical unrelated marrow grafts in lethally irradiated dogs by thoracic duct lymphocytes.

Characterization of host cells involved in resistance to marrow grafts in dogs transplanted from unrelated DLA-nonidentical donors.

A canine model of marrow transplantation was used to further define the host cells mediating resistance to marrow engraftment. Recipient dogs were given 9.2 Gy of total body irradiation followed by marrow infusion from unrelated DLA-nonidentical donors. No postgrafting immunosuppression was given. At three and ten days posttransplantation recipient marrow and peripheral blood cells were obtaine...

Synergism between mycophenolate mofetil and cyclosporine in preventing graft-versus-host disease among lethally irradiated dogs given DLA-nonidentical unrelated marrow grafts.

Mycophenolate mofetil (MMF) was evaluated either alone or combined with cyclosporine (CSP) for preventing graft-versus-host disease (GVHD) in dogs given 9.2 Gy total body irradiation and DLA-nonidentical unrelated marrow grafts. Marrow autograft studies showed gut toxicity as limiting MMF side effects. Four groups were studied for GVHD prevention: six dogs in group 1 received MMF 10 mg/kg twice...

Further studies on the role of circulating lymphocytes in the initiation of primary antibody responses to different antigens.

Thoracic duct cells obtained from normal (unimmunized) donors restored the primary hemolysin response of lethally irradiated or neonatally thymectomized rats to sheep red blood cells. Synergy between thoracic duct cells and bone-marrow cells was demonstrated in the irradiated hosts. However, thoracic duct cells did not restore the primary antibody response of irradiated rats challenged with dip...

DLA-nonidentical unrelated marrow grafts after high-dose total body irradiation: granulocyte colony-stimulating factor treatment after transplant does not enhance engraftment.

Late failure of autologous marrow grafts in lethally irradiated dogs given anti-class II monoclonal antibody.

We established a model of canine marrow autografts after 9.2 Gy total body irradiation (TBI) to study the role of class II antigens in hematopoietic stem cell growth and differentiation. Twenty dogs were given 9.2 Gy TBI, marrow, and intravenous (IV) murine anti-class II monoclonal antibody (MoAb). Infusion of 0.6 mg/kg/d of MoAb H81.98.21, an IgG2a reactive with HLA-DR, on days 0 to 4 after TB...